Comparison of Direct Sequencing with Real-time PCR High Resolution Melt and PCR Restriction Fragment Length Polymorphism Analysis to Identify Clinically Important Candida Species

Background: Candida albicans is the predominant yeast reported from human infection. Non-albicans species have been recently developed as medically vital fungi. Therefore, it essential to detect and identify pathogens at level prescribe appropriate treatment. Methods: This study assessed two complementary methods, including real-time polymerase chain reaction-high resolution melt (PCR-HRM) reaction-restriction fragment length morphism (PCR-RFLP) with standard PCR Sanger sequencing benchmark. Results: In total, 66 samples were tested, newly-advanced assays more effective displayed comprehensive concordance (66/66, 100%) outcomes. Moreover, accurate economical tests positively advanced by PCR-HRM for C. parapsilosis complexes. Conclusions: Given number of studies performed on comparison sensitivity specificity phenotypic genotypic methods diagnose invasive fungal findings this study, could be stated that correlative PCR-RFLP effectively substitutes conventional reasonable identification. However, supplementary evaluations confirming should carried out a broad range standardize method routine application in medical laboratories.